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Remarkably, the system was capable of discriminating between wild-type and mutant animals based on the differential viability of just six mechanosensory neurons.
We randomly chose 16 genes representing a range of base mean number from 4 to 3500 reads in both or in either N2 or wrn-1 mutant animals based on the RNA-seq normalized data (list of genes in Table 2) to determine the minimum of reads required for a gene to be detected by RT-PCR in our analyses.
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First, expression of the GABAergic specific marker unc-47 was unchanged in all mutants analyzed as compared to wild-type animals, based on the expression of the wyIs75 marker (Fig. 4A H).
DNA from fer-1 hc13);ced-3(n717) fer-1 hc13ant animals yields an approximately 10-base periodic ladder similar to that observed in apoptosis-competent fer- andoublestrains, indicating that CED-3 activity (and thus the canonical cell death pathway) is not required for the observed oocyte endocleavage.
Chi-square tests were used to determine whether the actual numbers of heterozygous and mutant animals born were statistically different from those expected based on the number of wild-type animals.
In all 11 mutant animals tested, none of them contained indels at 2 randomly chosen sites based on T7EI assay (Supplementary information, Figure S2).
We found that cho mutant animals occasionally survive to adulthood.
Members of the APPG were identified based on their induction by endoplasmic reticulum (ER) stress in mutant animals unable to mount a canonical ER stress response.
Based on these contradictory results, we considered the possibility that acs-20 mutant animals have a hyper-permeable surface barrier rather than increased lipid accumulation.
Homozygous dE2f1 mutant animals die during larval development (Duronio et al. 1995); thus, we used a dE2f1-dsRNA expression system based on the pWIZ vector (Lee and Carthew 2003).
These findings strongly suggest that the bone phenotype of the Cry2-deficient female animals is based on lowered osteoclast activity and hence is mechanistically different from the bone phenotype observed in Per2Brdm1 mutant animals.
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