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The abnormality was also apparent in mutant animals aged 8 months (n = 3) (data not shown).
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In females, the weight differences between double-mutant and Lepob/ob mice slightly increased as the animals aged (Figure 7B), similar to the growth patterns seen in Rcan2−/− and wild-type females fed the normal chow diet (Figure 2B).
Examination of coronal sections of Jna/+ mice (n = 6), littermate controls (n = 6), and rescued brains (n = 6) from animals aged 8 weeks revealed abnormal lamination of pyramidal cells in the hippocampus of mutant animals.
We compared the heart/body weight ratio of mutant animals at various ages with that of age- and sex-matched littermates.
As in the sectioned material, it was most apparent in the basal calyx of the MB in p35 mutants, and was observed only in aged, mutant animals.
The immunoblots of the testes of hsp90αgt/gt and wild-type animals showed a dramatic reduction in NASP levels in mutant animals, beginning at age P18 (Fig. 6, panel 5).
We therefore examined integrity of brain tissue in sections derived from wild-type or mutant animals at different ages.
Remarkably, this decline was much slower in the long-lived daf-2 e1370) daf-2 e1370that these mutant anindicatingathatuathesee-dependent reduction in cytochromutantontent.
The ogt-1 mutant animals appear to age prematurely, as exemplified by markedly slower movement at day 12 of adulthood relative to still-active wild-type and oga-1 mutant adults (Suppl. Movie 1).
The endosteal MAR is normal in 1 month-old mutant mice, but it is higher (+ 30%) in 2 month-old mutant animals compared to age matched controls (Fig. 5, panel E).
However, the E2f3 transcripts were highly expressed in the whole testes of mutant animals at the age of 10 12 weeks that coincided with a strong nuclear E2F3 staining of SC in IHC.
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