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(K,L) Sections show ZO-1 staining (arrows) at the reticular lamina of both control and mutant animals after the lesion.
Infarct size and lactate release from the heart were dramatically reduced in the mutant animals after I/R.
Indeed, the amount of cell corpses was decreased in ced-10 mutant animals after MG-132 treatment, thus confirming the proteasomal regulation of the CED-10 activity during the engulfment.
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On the other hand, five cell-cycle related genes appear to be up-regulated in mutant animals 24 days after birth.
To test this, we counted the number of fluorescent foci in hif 1 ia04) mutant animals expressing YFP polyQ35 after exposure to 1000 ppm O2.
Consistent with this observation, we did not detect any Rho1 protein by western blot or by indirect immunofluorescence of fixed embryos in Rho1E(br)246 mutant animals from 12 hr after egg laying (the earliest time point we could unambiguously identify mutant embryos; Figure 2C and data not shown).
Blood samples were collected from 1 and 2 month-old wild-type and mutant animals in the morning, after overnight fasting from tail vein.
In contrast to Rev-Erbα−/−Per1Brdm1 double mutant animals, triple mutants become arrhythmic after about 1 month in DD (Fig. 5B).
Larvae were generated by crossing homozygous to heterozygous mutant animals, and brains were genotyped after imaging.
These mutant animals develop severe jaundice soon after birth and die within 11 days, showing significant cerebellar alterations and the major features of severe neonatal jaundice (Bortolussi et al., 2012).
Strikingly, for some of these genes, the heat stress-induced transcriptional activation in the hrde- 1 mutant intensifies in the late generations under the heat stress and is heritable for at least two generations after the mutant animals are shifted back to lower temperature.
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CEO of Professional Science Editing for Scientists @ prosciediting.com