Sentence examples for mutant and selection from inspiring English sources

More than 250 plasmids were assessed by re-transformation of the mutant and selection for thermoresistance before analysis with restriction enzymes and sequencing.

Different display systems that allow construction of libraries of mutants and selection of binders with improved affinity, along with the gene coding for these variants, have been developed such as phage display or ribosome display systems (Hanes and Plückthun 1997; Bratkovic 2010).

To verify the degradation of the CL1 degron by autophagy, we generated a P. anserina strain lacking PaAtg1 and expressing Gfp-cl1 by crossing of single mutants and selection of the double mutant.

PaGlo1/ PaGlo2_OEx double mutants were generated by crossing the single overexpression mutants and selection of progeny in which recombination resulted in the combination of the PaGlo1 and PaGlo2 overexpression cassettes in one nucleus.

Open field day will be held each year for public touring the mutant plots and selection of interested mutants.

Nevertheless, maintenance of large mutant libraries and selection methods are difficult to implement in more complex systems such as mammalian cells and multicellular organisms.

Additional kanamycin was then added to bioreactors to concentrations typically used for mutant and plasmid selection (200 μg/ml), as well as a concentration representing only 25% of this level.

In "first-generation" msh1 mutants created by pollination of a wild-type line with mutant msh1 pollen and selection of the F2 msegregantsegregant, low-level recombination activity is initiated at each repeat.

The HeLa-tTA subline was subjected to a second round of transfection with constitutively active mutant GTPase plasmids and selection with puromycin, zeocin and dox.

After repeated cycles of mutagenesis and selection, mutant At 9 was generated.

The genetic optimization was carried out by random mutagenesis by ultraviolet irradiation and mutant selection by cerulenin, a compound displaying inhibitory effects on lipid biosynthesis.