Sentence examples for mutant and random from inspiring English sources

For assessment of c-FLIP mRNA expression, total RNA was obtained from cell pellets using Qiagen RNeasy mini kits (Qiagen, Doncaster, VIC, Australia) and reverse transcribed using M-MLV Reverse transcriptase (RNase H Minus, Point mutant) and random primers (Promega, Madison, WI, USA).

For mRNA analysis cDNA was randomly primed from 1 μg of total RNA following DNase 1 treatment (New England Labs) using M-MLV reverse transcriptase RNase H minus, point mutant (Promega) and Random primer 6 (New England Labs).

(E) Fluorescence polarization curves are shown for the binding of MrkH to the FAM-labeled mrkA promoter fragments (288 bps from −117 to +166; 80 bps from −117 to −37 and the 'MrkH box' mutant; and a random 80 bps DNA).

This method included fixed effects for mutant Pen-2 and random effects for a date within a given mutant (to adjust for multiple samples for some mutants on some dates) and for a set within a date (to adjust for a particular wild-type sample).

To investigate the role of the gp9 amino terminus, we have engineered a set of mutants with deletions and random substitutions in this part.

cDNA was generated from DNase I-treated RNA (2 μg total RNA) using M-MLV H-point mutant reverse transcriptase (Promega) and random hexamer or Oligo dT primers.

The frequencies of different types change according to the replicator equations and the fitness of a mutant is random and frequency dependent.

Screening of a library of random gene knockout mutants identified a mutant lacking TRPP2 (polycystin-2) that was resistant to the effect of naringenin on growth and random cell movement.

Pairing heterozygous mutant loci at random and testing for epistasis was conducted on yeast strains, in which rare and random mutations were induced by ethyl methanesulfonate (EMS) in a study conducted by Szafraniec et al. [ 173].

Punt et al. obtained a prtT mutant by random mutagenesis, resulting in a significant decrease of protease activity (Punt et al. 2008).

For one region of the GS FLX run (1/2 picotiterplate) of the mutant population, random subsets of sequence reads were generated with a custom Perl script.