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Here, we focused on one stronger mutant and one weaker mutant of streptavidin.
In later parts of this report, we focus on comparisons between one mutant and one wild-type for simplicity.
Twelve EGFR mutant and one ERBB2 mutant cell lines were analysed for LKB1 genotype.
One mutant and one littermate control animal were analysed from three separate litters.
Four days after irradiation, one mutant and one wild type were killed for histopathological analysis of the small intestine.
This feature was observed in both heterozygous (one mutant and one wild-type allele) and homozygous (two mutant alleles) animals.
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Microarray expression analysis was performed on parental cells as well as three independent knockout mutants and one heterozygously targeted clone.
Through screening of ~5000 M2 lines, we identified 10 Al-sensitive mutants and one Al-resistant mutant ral1 (resistance to aluminum 1).
To understand which residues in this WT 15-mer sequence are important for B56 binding, we generated 11 single-missense Ala mutants and one mutant with simultaneous mutation of the last four residues in this 15-mer sequence.
Site-directed mutagenesis was used to replace the amino acid residues of dalcochinase with the corresponding residues of Abg, generating three single mutants, F196H, S251V, and M369E, as well as the corresponding three double mutants and one triple mutant.
Five biallelic mutants, two heterozygous mutants and one homozygous mutant were obtained from 16 independent transgenic hairy root lines when the sgRNA was driven by the Arabidopsis U6 promoter, while no mutants were obtained from 13 independent transgenic hairy root lines when the sgRNA was driven by the rice U3 promoter.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com