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Total Chl and carotenoid contents were higher in the OPAO line than in fgl mutant and lower than in WT (Fig. 7e and g), which is consistent with their leaf phenotypes (Fig. 7d).
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Substituting Arg for Lys230 reduced activity only 3-fold compared with the 17-fold reduction in activity seen for the Lys230Glu mutant and lowered the bicarbonate Ka value while not changing the Asc Ka value.
It should be noted that in LCC2 there is a short poly Q containing mutant AIB1 and lower expression of the gene may be compensated by the increase in co-transactivation activity of the mutant protein.
CREB expression declined with age and was higher in young insulin receptor mutant worms and lower in young eat-2 worms and was maintained for longer with age in eat-2 mutants.
In separate analyses (to be submitted elsewhere), we tested the hypothesis of an irregular selection event to further explain the paradoxical observation of DP-cell accumulation in mutant mice, and lower yield of mature SP cells.
The analysis of the different genotypes of MBL showed a correlation between mutant genotypes and lower MBL serum levels as described before.
This demonstrates that, despite the mutant COG4 and lowered levels of several individual COG subunits, complexes can still be formed.
If they could find a molecule that would bind to the mutant CRYAB protein and lower its melting temperature back to that of a healthy CRYAB, they speculated, CRYAB should function normally and prevent damaged proteins from clumping in the lens.
However, the mutant proteins (middle and lower panels) show a clear shift from a plasma membrane to intracellular localisation and display a pronounced co-localisation with the Golgi marker GM130.
In each case, decreases in laccase activity were observed in ΔpdeL, ΔpdeH, and ΔpdeLΔpdeH mutants, with a less-oxidized dark purple stain around the colonies of the mutants and a lower level of the laccase activity in the culture filtrate compared with the wild-type strain (Figure 8A and 8B).
This shows that the retained levels of R165C frataxin are intrinsic to the expression or stability of this mutant, and that the lower levels observed of G130V and I154F may reflect altered entry into the mitochondria as proposed.
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