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Phospho-mimicking mutations at S635 and S1179 rescued the activity of the cytosolic mutant and increased those of the plasma membrane- and Golgi-targeted mutants.
As expected, transcription of α toxin was decreased in the Δstp1 mutant and increased in the Δstk1 mutant.
These mutations also increased protease sensitivity relative to the fcho-1 mutant, and increased phosphorylation of threonine-160.
The pde2Δ mutant was as sensitive to NaCl as the sir2Δ mutant and increased the Pma1 mRNA level as high as the sir2Δ mutant.
It has been shown that rhlA, encoding a homologue of Pseudomonas aeruginosa RhlA involved in rhamnolipid biosynthesis, showed decreased expression in an hfq mutant and increased expression in a rsmA mutant [ 12, 17].
WRKY33 also bound to the CYP707A3 promoter, a gene involved in ABA catabolism (Leng et al., 2014), but its expression decreased in the wrky33 mutant and increased in WT plants after infection indicative of positive regulation by WRKY33.
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To ensure accuracy of 35S::OsNCED3 molecular construct, 35S::OsNCED3 was heterologously transformed into Arabidopsis and the overexpression of OsNCED3 was able to complement the ABA-deficient phenotypes in the 129B08/nced3 mutant and increase ABA content in wild type (Hwang et al. 2010).
As expected, total dendritic length was severely decreased in cul1 mutants and increased in cul3-mutant ddaC neurons, when compared to wild type (Figure 5B).
The expression of AtCNGC1 in K+ uptake deficient mutants of yeast and Escherichia coli enhanced growth of these mutants and increased intracellular [K+].
These findings suggest that QRDR mutations in P. aeruginosa play an insignificant role in the increased MICs of orbifloxacin against the mutants and increased MPC of orbifloxacin.
In tomato, lycopene content is greatly reduced in the fruits of JA-deficient mutants and increased in transgenic lines with enhanced JA levels.
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