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This analysis revealed that pri-miR172a and pri-miR172d transcripts were elevated in the osphyB mutant and further increased in the osphyA osphyB double mutant (Figure 4C and D).
Proteolytic activity was reduced in the AB1.13 mutant and further reduced to about 10% of the wild-type level in the D15 mutant (Table 2).
The present study reports identification and characterization of a Drosophila P32 mutant and further defines the molecular basis of mitochondrial function in neurotransmitter release.
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Essential subspace overlap analyses were performed to quantitatively distinguish the preference for conformational transitions between states of the gp120 mutants and further to ascertain what kind of conformational state that the mutants prefer to adopt.
We and others have shown neural tumors are sensitive to oncolytic Herpes simplex virus (oHSV) mutants and further hypothesized that these viruses may effectively destroy drug-resistant neuroblastoma tumor initiating cells [34], [35], [36], [37], [38], [39].
These findings indicate that the miR-155-inducing activity of the LPS is impaired in the GGT mutants and further suggest bacterial GGT activity plays a supportive role in LPS-dependent miR-155 expression.
Thus, HR is essential in sen1-1 mutands, and further inactivation of the Sgs1 or Srs2 DNA helicases leads to the accumulation of lethal recombination intermediates.
To confirm the reduction of ptCpn60β proteins in ptcpn60β mutants and further examine localization of the proteins, we prepared antibodies using recombinant ptCpn60β1.
Next, we performed the same reporter gene assay using different Tip110 deletion mutants and further examined the structure function relationship of Tip110 in terms of pre-mRNA splicing.
The usefulness of the mutagenesis tolerance assay in screening reporter genes was confirmed with the usage of these tandem nucleotides in mutants and further mutants with a series of specific sequences.
Together, these data show that NiTrac1 is a sensitive tool for reporting conformational changes in mutants and further support the hypothesis that NiTrac1 reports activity states of the transporter.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com