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Complete concordance for factor V Leiden genotypes was observed between the two methods in the 205-sample cohort comprising 139 wild-type, 62 heterozygous mutant, and four homozygous mutant individuals.
We counted epithelial cells in eight mutant and four wild-type ears and found a significant difference between the percentage of cells positive for pSmad2 (P = 0.00029036).
To validate proteomic data, protein extracts from an independent set of biological replica made of pooled samples from four Ugt1 mutant and four WT mice were loaded onto 7 cm IPG strips, separated by IEF and SDS-PAGE and then transferred overnight to nitrocellulose support (Schleicher & Schuell, Keene, NH, USA).
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Six of the cell lines (NOMO-1, HEL, HL-60, SKM-1, THP-1, and TF-1) are TP53 mutant and three of the cell lines (EOL-1, MOLM-13, and MV4-11) are TP53 wild-type.
a Col-0, 129B08/nced3 mutant, and two N4C transgenic lines after 9 days of withholding water.
b Col-0, 129B08/nced3 mutant, and two N4C transgenic lines after 13 days of withholding water.
Fig. 2 Phenotypic comparison of 35-day-old, soil-grown Col-0, 129B08/nced3 mutant, and two OsNCED4-overexpressing transgenic lines.
c RT-PCR of REL2 in rel2 mutant and three independent complementary lines c-rel2-1, c-rel2-2 and c-rel2-3.
Fig. 1 RT-PCR analysis of native AtNCED3 and transgenic OsNCED4 expression in Arabidopsis plants; Col-0, 129B08/nced3 mutant, and two OsNCED4-overexpressing (N4C) transgenic lines.
a ABA content in seedlings of Col-0, 129B08/nced3 mutant, and two N4C transgenic lines grown on agar medium supplemented with 0.2 or 4% Glc for 10 days.
But heterozygotes, with one mutant and one normal Kit gene, do fine, although their feet and the tips of their tails are white rather than gray.
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