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We thank Dr. Bernard Roizman for providing the ICP0 mutant and for generous support for the project.
The SASA for residues M205, M206 and M213 was calculated by averaging along the whole simulation time for each mutant and for the wt protein.
Most differentially regulated genes of the single mutants were also differentially regulated in the double mutant, and for the most part in the same directionality.
The luciferase reporter constructs UAStkLuc and PPREtkLuc, as well as plasmids for Akt and its inactive K179M mutant, and for constitutively active p110α catalytic subunit of PI3-K have been described [26], [44].
For SUMO1, the proportion of cells in which we observed colocalization decreased to less than 30% with the ΔGG mutant, and for SUMO2 colocalization was effectively abolished (Figure 6D and E).
Fig. 6A shows fluorescence vs. voltage relationships for the fs-α1SM701 fs-α1SM701d for thIs mutant coexpressed with α1SΔ1–700.
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The importance of accurate estimation of the cell growth rate is more eminent for the dynamic simulation of the specific gene knockout mutants and for the effect of culture condition.
For each of these mutants and for wild type cells we established clones expressing CFP-ubiquitin, mRFP-PCNA or both.
This MAb exhibited only minor reactivity reductions (50 25%) for all six G106/L107 fusion peptide mutants and for two individual EDIII mutants E311R and P364R (50%).
Their offspring until F5 were crossbred again and genotyped for E- and P-selectin-null mutants and for scid.
Less strong (but nevertheless significant) decreases in thioacidolysis yield were noticed for both f5h1 and comt mutants and for c4h-2.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com