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T. denticola Cpt complemented a Saccharomyces cerevisiae CPT1 mutant, and expression of the entire T. denticola LicCA-Cpt pathway in E. coli resulted in phosphatidylcholine biosynthesis.
Early onset tumor formation in MMTV/Wnt1 mice is significantly delayed by expression of a dominant-interfering PEA3 mutant, and expression of this dominant negative PEA3 transgene is markedly reduced in tumors relative to non-tumorous mammary tissues.
The KlROX1 gene on a 2μ-based vector was then transformed into the S. cerevisiae Δrox1 mutant, and expression of KlRox1p was confirmed by Western blot (data not shown).
Although many aspects of the maize SAM have been studied through mutant and expression analysis, an understanding of the genetic control of natural variation in SAM architecture is unknown.
We added a detailed molecular characterization of the mutant in Figure 5 figure supplement 1. Additionally we show some data on the phenotypic appearance of the mutant and expression of selected AKIN10 target genes in the mutant in light and extended night.
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All conditional knockdown mutants were TetON mutants and expression of the targeted gene was induced with anhydrotetracycline (atc) or doxycycline (doxy) and repressed without atc/doxy.
Molecular studies of bacterial virulence are enhanced by expression of recombinant DNA during infection to allow complementation of mutants and expression of reporter proteins in vivo.
Adaxial cell expression is absent in yot mutants, and expression is also regionally lost in the CNS.
Remarkably, DAPT restored ascl1b expression in hdac1 s436 mutants, and expression was maintained at least up to 48 hpf (compare Fig. 2C and Fig. 4A).
Loss of Marf in RG recapitulates the bouton phenotype observed in Marf mutants and expression of Marf in RG fully rescues this phenotype.
Deletion mutants and expression profiling of genes in the region containing the Ph1 locus on 5B has further restricted Ph1 to a cluster of cdk-like genes.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com