Sentence examples for mutant and determined from inspiring English sources

To verify the relevance of in-silico predictions, we constructed a PGAA + Phe24βCys mutant and determined its enantioselectivity in biocatalytic reactions.

We had also tested the GSS-associated A117V mutant and determined that this mutant retained its anti-Bax function in MCF-7 cells and in human neurons.

Therefore, we infected macrophages with wildtype M. marinum and the mptC mutant and determined intracellular replication and intercellular spread.

We have constructed and characterized a strain carrying a null allele of sltB, shown that its phenotype is indistinguishable from that of a null sltA mutant and determined that the two null mutations are non-additive, indicating that sltB is an element of the sltA stress response pathway.

To determine if the Tyr side chain position is the sole determinant of the isoform-dependent inhibitor binding mode, we have prepared the eNOS Y477A mutant and determined crystal structures of the mutant bound with two inhibitors that do not disturb the Tyr477 rotamer in wild type eNOS.

Though not essential we suggest the following – either for this paper or for a discussion of future directions: reconstitute pol V Mut with a RecA mutant that can no longer bind ATP (e.g., less conservative mutant) and determine if it still binds etheno-ATP, and to use the same mutant in ATPase assays.

Similarly, we made RIG-I deletion mutants and determined which domain is required for interaction with REUL.

In addition, we performed molecular dynamics (MD) simulations on the mutants and determined the crystal structures of PDE9Q453E in complex with the inhibitors 3-isobutyl-1-methylxanthine (IBMX) and (S -BAY73-6691 (Fig. 1).

We examined ovaries from the different spn-E mutants and determined whether dynein aggregates form by immunohistochemistry using EGL as a marker for the aggregates.

To further study the functionality of S231, we transfected the human cancer cell line HCT116, which allowed high transfection efficiency, with the S231D and S231A Par-4 mutants and determined their effect on apoptosis.

We have also used inverse splinkerette PCR to map insertions in 38 mutants and determined approximate location of the insertion vector in 14 mutants, nine of which we were not able to determine by TAIL-PCR (Additional file 2: Table S1).