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To assess the genetic epistasis between Desi3a and FLS2 upon flagellin perception, we analysed flg22-mediated root growth inhibition of fls2 double-1 double mutant, and compared it to Col-0, fls2 and desi3a-1 single mutants in the presence of 250 μM flg22 (Fig. 4a, b).
APTX-mediated DNA deadenylation activity (i.e., 5′-AMP removal) was measured in extracts of cells expressing wild-type XRCC1 or the XRCC1 phosphorylation mutant, and compared with activity in APTX-deficient and APTX-complemented human cells.
We generated a NEMO K285R mutant and compared TRAF6-mediated modification of NEMO in the presence or absence of lysine 285.
To validate this approach we sequenced the genome of Bdellovibrio bacteriovorus 109J, an obligatory bacterial predator, and its prey-independent mutant, and compared both to the mediator species Bdellovibrio bacteriovorus HD100.
The corresponding males were noted Atp6ap2 Camk2aCre/0 or Atp6ap2 Cask2aCreERT2/0 as mutant and compared with wild-type littermate (wt) as controls.
Km and Vmax values were obtained for one mutant and compared with wild-type values obtained in the same set by unpaired Student's t-test.
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The genetic basis for phenotype alteration can be identified directly by sequencing the entire genome of the mutant and comparing it to the wild-type (WT) genome, thus identifying acquired mutations.
Finally, we analysed the interaction energy profiles of the mutants and compared them to the wild type protein using Discovery studio 2.5.
In one experiment, the scientists electrically stimulated nerve cells from the mouse brains, assessed the strength of synapses from Shank3 mutants and compared these to brain tissue from normal, wild-type mice.
Therefore, we examined the rate of germination in representative color mutants and compared it to that of wild type.
Using AutoEPG we analysed specific features pertaining to the shape of the EPG waveform of slo-1 js379 slo-1 js379d comutantsthem to the wild-type.
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