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By combining expression studies, mutant mice, and cell models, we have now characterized the roles of miR-34a in the mammary epithelium.
But you can't say mutant and normal cells respond to the chemical differently: to say that, you would have to do a third statistical test, specifically comparing the "difference in differences", the difference between the chemical-induced change in firing rate for the normal cells against the chemical-induced change in the mutant cells.
Our analysis of compound E2f4:pocket protein mutant mice and cells indicates that the E2F4/pocket protein complexes play a key role in the repression of E2F-responsive genes.
This new hypothesis is used to explain the variability in radiosensitivity observed at various cell-cycle-stages, among mutant cells and cells of different origin, or among different individuals, and to revisit unresolved issues and unanswered questions.
Upon release from G1, wildtype, rad61 and ctf18 single mutant cells and rad61 ctf18 double mutant cells and also ctf7 rad61 ctripleriple mutant cells all exited S-phase in synchrony such that the time interval from G1 to mid-replication and then to G2/M accumulation is nearly identical (Figure 4).
C.E., S.H., and I.G.B. generated the H. pylori MreC mutants and performed cell growth and microscopy experiments.
Analysis of PI-PLC in g alpha 2 null mutants and in cell lines expressing mutant G alpha 2 proteins also strongly suggests that G alpha 2 is the G alpha subunit that directly activates PI-PLC during aggregation.
Serial dilution of log phase smc3Q and ctf18 single mutant cells and smc3Q ctf18 double mutant cells were plated onto rich medium and challenged at a range of temperatures.
We have analyzed both mutants using biochemistry and cell growth.
