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Gln3-Myc began relocating to the cytoplasm immediately and was completely cytoplasmic within 10 min. The first mutant analyzed was a gtr1 Δ (FV406).

eSDC values, and SHAPE data for all mutants analyzed are provided as tables in the additional files.

To ensure that all mutants analyzed were independently generated, we isolated only one and, occasionally, no more than two well-separated mutants per dish for analysis.

Exposure to an equitoxic dose of alpha radiation from 212Bi, a 220Rn daughter, resulted in a spectrum similar to the gamma-ray spectrum in that 75% of the 49 mutants analyzed were deletions.

Most of the dumpy mutants we analyzed were generated by EMS following Jenkins [13] in stocks isoallelic for dumpy.

The observation that∼50% of the conditional mutants we analyzed are potentially fast-acting provides additional incentive to isolate more TS alleles.

The deletion mutants we analyzed are shown in Figure 2a.

Certain of the Ts– mutants we analyzed were defective at high temperature specifically in the assembly of new rings.

Among the five different parkin mutants analyzed, AF-6 was only able to partially increase the translocation of the T240R mutant (Fig.  8A C).

However, the mutants analyzed here are hypomorphs and the allelic combination used, dge01554/dg323, is viable.

Categorization of all 264 mutants analyzed by aCGH is documented in Table S2.