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The genetic characterization of Delta toxin will be useful for further epidemiological studies and mutant analysis to address the involvement of this toxin in pathology.
For example, Notch signaling has been shown by mutant analysis to be required for the proper specification of the wing margin [17].
We have added mutant analysis, to complement morpholino data, wherever possible.
Extensive double and triple mutant analysis to find regulators of cell death has been done in the background of acd6 and syp121syp122[ 46, 101].
Despite these limitations, Drosophila geneticists have successfully used mutant analysis to identify loci integral to behaviors such as learning and memory, circadian rhythmicity, foraging and reproduction [Reviewed in [ 1]].
We used whole organism RNA seq combined with mutant analysis to identify genes regulated by the MAB-5/Hox transcription factor in Q neuroblast descendant anterior-posterior migrations in C. elegans.
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We then looked for the interacting partner(s) of residue 22 by determining the potency of block of Y401K, Y401A, E758Q, D762K, D762A, E765K, E765A and D1241K channels by WT μ-CTX and A22E, followed by mutant cycle analysis to assess their individual couplings.
A remaining challenge, undertaken in this study, is to link mutant cycle analysis to extended networks.
The subtle difference between GA and GB (which only differ by a single hydroxyl group at position 1; Fig. 1 A) allowed us to use mutant cycle analysis to probe for direct interactions with the 2′ residue (24).
Genetic analyses show that FLN1 plays a more dominant role than FLN2; single mutant analysis showed FLN1 to be required for autotrophic growth; double mutant analysis revealed that complete loss of FLN function results in seedling lethality even on an exogenous carbon source.
Non-virulence roles for mycolic acids, mostly inferred from mutant analysis, include resistance to antibiotics, ultraviolet and γ-irradiation, oxidative stress, various chemicals, desiccation and regulation of biofilm formation (Brennan and Nikaido, 1995; Ojha et al., 2005).
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