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Mutant analysis is a useful approach to illuminate the function of gene in complex biological processes of chloroplast development.
As already reported, the use of hypomorphic alleles in the study of epistasis and double mutant analysis is problematic in the nematode.
One explanation for this difference between the RNAi result and the double mutant analysis is that the dnc-1 (or404 ) allele is not a null.
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Similarly, the reviewers found that the mutant analysis was not fully developed.
Identification of gish as a para bss1/+ BS suppressor by mutant analysis was supported further by RNAi analysis.
Given the high UV sensitivity of polh-1 mutants, less than one-tenth the UV dose used in NER or the smc-5 mutant analysis was applied.
The genes in this cluster (Additional file 9: Table S2) included genes that in our mutant analysis were important in lesion formation, including SID2, PAD4, WRKY25 and WRKY33.
Genetic mutant analysis was used to investigate and compare the in vivo functional contributions of AGP glycosylation by GALT3, GALT4, and GALT6 with that of GALT2 and GALT5 (Fig. 5; Additional file 1: Table S1).
3) Some of the mutant analysis was done with an assay using a large fusion protein consisting of MBP fused to the Sna3C peptide by a TEV cleavage site.
Comparisons of the genetic effect of the E2 allele in three different experiments supports the stability of the E2 allele because the additive effects observed in Tsukuba, in Sapporo, and in the mutant analysis were 5.2, 4.3, and 3.9 days, respectively.
The mutant VEGFR (kdrl) analysis is more convincing, but again, are there other genes or isoforms that might function redundantly with the kdrl receptor?
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