Double mutant analysis demonstrates that PAX1 interacts genetically with other members of the Aux/IAA family in addition to AXR3.
Mutant cycle analysis demonstrates a strong interaction between the ginkgolides and the 2′ residue, a result supported by in silico docking of compounds into a model of the pore.
These studies include incisive double mutant cycle analysis demonstrating functional and energetic connections between residues (Hermes et al., 1990; Dion et al., 1993; Horovitz et al., 1994; Rajagopalan et al., 2002; Masterson et al., 2008; Singh et al., 2014).
Furthermore, mutant analysis has demonstrated that loss of Pcdh10 can influence different aspects of development and post-natal life [ 48].
Quantitative analysis demonstrated that mutants containing p27T198A/G displayed a half-life of about 2.5 hours respect to the 7 9 hours of p27T198V, p27WT or p27T198E.
For the pdh2-1 mutant (in L er background), segregation analysis demonstrated the presence of a single transposon.
Furthermore, blood analysis demonstrated that male mutant mice had significantly elevated blood insulin compared with controls, with female mice showing a large difference approaching significance (Fig. 4D).
This analysis demonstrated that the mutant MEFs show significant defects in basal respiration, oligomycin-sensitive respiration and total respiratory capacity (Fig. 4C; supplementary material Fig. S5).
BrdU incorporation analysis demonstrated that the mutant BRCA1 transgene (but not siRNA treatment) inhibited S-phase progression in both T47D (17% versus 10% positive cells; p < 0.03) and MDA-MB-468 (20% versus 16%; p < 0.02) lines (Fig. 4a).
STD-NMR analysis demonstrated that the mutant protein could bind to both the Lewis-y tetrasaccharide 7 and also the B-Lewis-y pentasaccharide 8. Furthermore the STD-NMR data indicated that each oligosaccharide binds in a similar orientation to the complexes with LTBh.
