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Here, we performed expression and mutant analyses to identify the upstream regulator of miR156.
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Additional experimental methods, such as double mutant analyses, may help to elucidate networks of direct transcriptional control [ 14].
Further studies using both ribosomal profiling as well as specific mutant analyses are required to advance our understanding of these novel regulatory mechanisms.
These sorts of analyses may also be used much in the same way as mutant analyses are used to identify genes involved in a specific process (i.e., R or S).
Similar Glu homeostasis was observed in many studies on nitrogen nutrition, environmental stress or mutant analyses and was proposed to indicate a special regulatory function of Glu levels [ 44].
pAbp mutants display cytokinesis defects, and mutant analyses demonstrated that pAbp is required to connect the centrosome to the minus-ends of free microtubules (reviewed in [ 47]).
According to the mutant analyses (Ishihara et al., 2007), PPL1 is an efficient photo-damage repair factor of PS-II, and thus the interaction between PPL1 and PS-II is probably transient.
In agreement with the results of the present study, recently, it has been shown via mutant analyses that Arabidopsis histone chaperones belonging to ASF1 family play a role in transcriptional activation in response to heat stress [ 50].
Double mutant analyses showed that EDM2 is epistatic to WNK8.
This is in contrast to IXB, where mutant analyses have identified specific cellulose synthase (CesA) complexes (CesA3 and CesA6) from the plasma membrane as toxin targets [ 8, 9].
For Picciotto vs Xu mutant analyses the minimum fold change was increased to 2.0.
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