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Here we analyzed the energetic contributions of the residues from these two approaches by single and double mutant analyses and determined crystal structures for a variant from the calculation (I16/L18/E25/K29) and from the selection (I16/I18/E25/F29).
Mutant analyses and C NMR studies confirmed GB catabolism in P. aeruginosa[ 129].
Here we investigate the nature and relevance of visceral homeostasis in Drosophila by developing an integrative method to quantify metabolic features, which we combine with mutant analyses and the selective manipulation of neuronal subsets.
Similar Glu homeostasis was observed in many studies on nitrogen nutrition, environmental stress or mutant analyses and was proposed to indicate a special regulatory function of Glu levels [ 44].
The Pi regulatory mechanism has been elucidated through Arabidopsis mutant analyses and involves PHR1 (phosphate starvation response 1), a MYB-type transcription factor, acting as a key factor in regulating downstream –P-responsive gene expression, including that of IPS1, through the P1BS cis-acting element (GNATATNC) of the PHR1 binding site.
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Over-expression and mutant analyses of CBLs and CIPKs have greatly enriched our understanding of their functions.
During wildtype embryogenesis, both genes are expressed during neuroblast delamination, and mutant analyses suggest that Snail and Worniu function in neuroblasts, around the time of division to give rise to ganglion mother cells (GMCs) [ 61].
Their PGM activities, shown both in this study and previously [6], coupled with our mutant analyses demonstrating overlapping and supplementary functions in the cell unequivocally establish the two forms as NISE.
We used the temperature-sensitive or404 allele of dnc-1 in the double mutant analyses with mel-28, and we moved L4 animals to 25° for analysis.
Gene expression and mutant analyses showed that LLG1 is important for vegetative growth and development.
Extended Data Fig. 3 Transcriptional and mutant analyses of HD-ZIP III transcription factors.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com