Sentence examples for mutant along a from inspiring English sources

In total, 62 regions (17 SSR, three SNP, one CAPS and 41 re-sequenced gene fragments) were scanned for polymorphism both in Chardonnay and Ugni Blanc mutant along a 3.4 Mb interval (85,127-3,506,060 bp) in the flb region (scaffold 122 and the beginning of scaffold 1).

An extremely compromised activity of K487A mutant along with a tremendously low Kcat value (Table 2) suggests a crucial requirement of Lys487 for the enzymatic activity, which agrees well with the role of the cognate residues in other homologous proteins [24], [25].

Similar values were obtained for the other HOG pathway mutants, ste20Δ (−0.36) and ste50Δ (−0.50), showing that FVI data of mutants along a pathway are in good agreement (Fig. 1C).

To measure the fitness of HIV-1 RT mutants along an AZT resistance pathway and to evaluate the effect of epistasis ( =  the fitness interactions between the mutations), we generated the respective RT mutants by site-directed mutagenesis PCR [51].

To determine the role of each residue of AChE586-599 in the process of amyloid formation, a library of alanine scanning mutants along with a structurally conserved substitution mutant (Tyr to Phe) and a truncation mutant (missing the last residue) were used.

We report 7mG excision and DNA binding activities of AlkD mutants, along with a comparison of alkylpurine DNA glycosylase structures.

These mutants, along with a second vortex mutant and another mutant named thoraxate showing thoracic vortices and homozygous lethality, were eventually combined by Bridges and Muller as a series of recessive allelomorphs possibly occurring in different parts of a single gene [6] [8].

For libraries sequenced prior to screening, one example of each codon was assayed; when individual sequences were unknown, we assessed 95 randomly chosen mutants along with a wild-type control.

In contrast, a triple mutant along the Ubl-binding surface of helix 1, D262A/L266K/D274R, is unable to form ubiquitin chains or ubiquitinate Miro1 (Fig 6C).

To investigate the role of phosphorylation on BCA2 stability, HEK293T cells were co-transfected with a constitutively active variant of AKT or a kinase-dead AKT mutant, along with FLAG-tagged BCA2 or the S132, 133A mutant which abolished AKT phosphorylation.

These mutants along with an expanded mutational analysis in IPKs from mesophilic hosts can serve a variety of applications.