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Finally, the application of constraints corresponding to different environmental conditions (e.g. minimal growth medium) or different genetic background (e.g. enzyme-deficient mutant) allow the transition from metabolic network reconstruction to condition-specific model.
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The ΔglcD1 mutant allowed the demonstration of enhanced carbon flux into the glycolate pool after a shift to lowered Ci during the chase phase (Figure 3).
A positive fitness effect of homozygosity for the fertilization mutant allows the mutation to spread more rapidly through the population.
The use of the estrogen receptor mutant ERTamoxifen Mutant allows the use of the synthetic ligand, 4-hydroxytamoxifen (4OHT) for induction.
Presumably the P14V/P15G mutant allows the α- 4,5-dimethylfuran-2-yl) substituent of 8 d to more readily adopt the co-planar orientation required to stabilise the transition state leading to the enediolate intermediate.
As explained above for the 3xK mutant, because the phosphate group has a negative charge, the K624E residue may sit with the phosphate group driving the Aβ production more like the noncharged mutant allowing the construct to sink a bit further down than the WT (single lysine).
Heterozygous mutants allow the effects of dominant alleles to be observed.
However, these mutants allow the analysis of endogenous substrate pathways and the expression of hydrogen metabolism associated genes under steady state growth conditions without the background of nutritional stress.
Approaches using spontaneous and induced mutants allow the characterization of the complex underlying gene expression system integrating carbohydrate, amino acid, and storage protein metabolisms, and operating during endosperm growth and development.
Reference metabolites produced by P450 Bmutantsnts allowed the characterisation of activity and regioselectivity of metabolism of all three NSAIDs by thirteen recombinant human P450s.
Homozygous mutants allowed the testing of IgE binding on the altered proteins from seed extracts.
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