Shortening in cn/cn mutants affects both axial and appendicular portions of the body and proximal, middle, and distal parts of each limb.
These mutants affect both exposed and non-exposed cilia, suggesting that both classes of neurons are involved in sensing NH4Ac.
Further, establishment of the P cytotype by telomeric P insertions was shown to be sensitive to mutants affecting both heterochromatin formation (HETEROCHROMATIN PROTEIN 1, "HP1") and small RNA silencing pathways (AUBERGINE, an Argonaute member), suggesting a complex molecular mechanism [25], [30], [31].
This was made possible by combining this highly specific TSS determination technique with the use of mutants affecting both the nuclear exosome and the cytoplasmic NMD pathway.
In summary, with the DsDELGT4 system, we have successfully obtained numerous tissue-specific transgenic lines and created mutants affecting both known and novel genes.
In contrast to the Nup53 mutants and truncations that abrogate one membrane binding region, mutants affecting both membrane binding sites (1 319 R105E/K106E, 1 319 S94E/T1 312 1–312 R105E/K106E and 1 312 S94E/T100E) did not support NPC assembly and NE reformation.
Although am1 mutants affect both male and female meiosis, anthers were chosen because they are much easier to dissect than the female floral tissue and because comprehensive transcriptome studies of normal maize anther development are already available on this array platform [ 20- 22].
One possibility is that each mutant affects different aspects of SPB duplication.
(A ) luchs t32241 (luc ) mutant affects Cx39.4 and results in meandering and broken stripes.
However, the A98V mutant was almost absent from the void volume, suggesting that this mutant affects ring formation.
