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However, mutant RNPs generated with a viral CAT replicon showed up to hundred-fold reduced CAT accumulation.
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The activity of each of these rescued mutant RNPs was again tested in the CAT minireplicon assay as described above and the results are presented in Fig. 6B.
These results indicated that there is a correlation between the progressive reduction in mutant PB2 domain interaction with importin alpha [10] and a decrease in the activity of the mutant RNPs.
We identified a rice small grain (sg2) mutant in an EMS mutant library generated from an indica variety, Shuhui498.
viciae AapQ site directed mutant was generated as follows.
In parallel, a constitutively dimeric mutant was generated (R227E/K228E) [36].
The deletion mutant thus generated was named STMΔhfq.
Hence, we conclude that a true pilC1/C2 mutant was generated.
Mutants on the ribH1, ribH2 genes and a double ribH mutant were generated.
The SC/09 mutant was generated by reverse genetics.
The saw1-K221R mutant was generated by site-directed mutagenesis.
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