Sentence examples for mutant PMLs generated from inspiring English sources

The mutant PMLs generated are truncated and do not have a nuclear localization signal (NLS) [ 91].

The methanol tolerance of wild-type and mutant PMLs as a function of methanol concentration is shown in Figure  5A.

Large crystals of the mutant PML formed in many conditions between one day and two weeks.

Over-expression of wild-type and mutant PML was carried out in E. coli BL21Gold DE3) (Agilent).

Ectopic overexpression of wild-type PML, which forms PML-NBs, significantly rescued the resistance to H2O2-induced cell death in SIRT1 knockdown HeLa cells, but not the cytoplasmic mutant PML (K487R).

Interestingly, we found that mutant PML (K487R) is devoid of SUMO1 conjugation, but NLS-K487R is SUMO1 conjugated.

To construct NLS fusion PML protein, NLS derived from the simian virus 40 (SV40) large tumor antigen (PKKKRKV) was added at the N-terminus of HA-tagged wild-type and mutant PML protein.

Figure  5B shows the residual activity of wild-type and mutant PML from the different rounds of screening after treatment with 50% methanol as a function of time.

We found that the SUMO1 conjugation at K490 was significantly increased in mutant PML (K65 and K160), whereas SUMO1 conjugation at K65 and K160 was almost undetectable.

In order to determine if the mutant lipases were also more resistant to methanol inactivation over a long period of time, wild-type or mutant PML was incubated for 24 hours in the presence of 50% methanol.

A highly thermostable mutant lipase was generated and characterized.