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Both control and dlg X 1 2 mutant NMJs contain highly visible clusters of GluRIIA-containing receptors (Fig 2A; magenta).
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While control NMJs contain numerous mitochondria per bouton, Marf boutons contain almost no mitochondria, even fewer than in Drp1 mutants.
Drosophila embryonic/larval NMJs contain two molecularly, biophysically, and pharmacologically distinct subtypes of postsynaptic glutamate receptor: 'A-type' and 'B-type'.
Inhibitory NMJs contain both mobile and immobile UNC-49/GABAA receptors.
The GABAA receptors found at these NMJs contain two subunits (UNC-49B and C) both encoded by the unc-49 gene (Bamber et al., 1999).
Drosophila NMJs contain two spatially, pharmacologically and biophysically distinct subtypes of postsynaptic glutamate receptor [ 38- 42], referred to as 'A-type' and 'B-type' receptors.
In other words, the Drosophila NMJ contains two subclasses of ionotropic glutamate receptor: 1) GluRIIA-containing receptors and 2) GluRIIB-containing receptors.
Miniature postsynaptic potentials are larger in larval neurexin mutant NMJs [37], [39].
In embryos, decreased neurotransmission in neurexin mutant NMJs appears entirely attributable to the decrease in NMJ active zone number that we observed (Fig. 5).
This might indicate a link with the reduced accumulation of Glutamate Receptors at the mutant NMJs in the mutant animals.
Together these data provide evidence of defects in both the pre and post-synaptic components of the mutant NMJs.
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