Sentence examples for mutagenesis which introduces from inspiring English sources

In contrast to QuickChange™ site-directed mutagenesis, which introduces single mutations or small insertions/deletions, RF cloning inserts complete genes without the introduction of unwanted extra residues.

We previously screened mutant mice generated by N-Ethyl N-nitrosourea (ENU) mutagenesis, which introduces single base-pair changes.

Truncation provides the advantage of not disrupting the first layer of recognition of the protein, thus keeping intact the original binding pocket, in contrast to site-directed mutagenesis, which introduces a different amino acid.

Site-directed mutagenesis is a novel molecular biology tool, which introduces mutations into DNA fragments of interest in a well-defined manner.

Five different mutant forms of p53 were introduced by mutagenesis which are known to be associated with reduced protein stability (V143A), defect of DNA binding (R248W, R273H) or structural instability (R175H, R249S) [ 16].

Error-prone polymerase chain reactions (epPCRs) are often used to introduce mutations in random mutagenesis, which has been used as a tool in protein engineering.

One strategy to overcome this issue is provided by site-directed mutagenesis, which enables a single nucleophilic cysteine residue to be introduced at a desired site in an antibody.

This indicates that high XI activity is indeed beneficial for D-xylose fermentation but that it requires one or more mutations in the genome of the strain, which were introduced during the mutagenesis procedure, to be effective.

pcDNA3.1-CTBP2-TT expresses CtBP2 with a C-terminal tandem affinity purification tag; the plasmid also contains a synonymous mutation of the target region of the CtBP1/2 siRNA, which was introduced by site-directed mutagenesis.

A vector encoding a modified β-conglycinin α′ subunit (4novokinin-α′) in which four novokinin sequences have been incorporated by site-directed mutagenesis was introduced into somatic embryos by whisker-mediated gene transformation to produce a transgenic soybean.

The S. aureus NAL which contains no natural cysteine residues was subjected to site-directed mutagenesis to introduce a cysteine in place of Lys165 in the enzyme active site.