Sentence examples for mutagenesis were utilized from inspiring English sources

In this work, error-prone PCR and site-directed mutagenesis were utilized to improve the thermostability of nitrilases.

Site-directed mutagenesis, random mutagenesis, and site-saturation mutagenesis were utilized to increase the OP-degrading activity of Dr-OPH.

Chromatin immunoprecipitation (ChIP) and site-directed mutagenesis were utilized to determine how Pokemon regulates survivin expression, a target gene.

The expanded saturation mutagenesis was utilized to evaluate the effect of a series of atomic-level side chain substitutions on the protein activity.

Site-directed mutagenesis was utilized to create the GFP-MSH6 mutations described.

Site directed mutagenesis was utilized to probe its functionality by mutation to either an alanine or a lysine (R288A or R288K, respectively).

Site directed mutagenesis was utilized to probe changes in activity upon mutation to either an alanine or an aspartate (E105A or E105D, respectively).

Site-directed mutagenesis was utilized to mutate a Flag-Tim-1 construct, using the QuikChange system (Stratagene).

To better demonstrate in vivo whether the direct zinc binding to Tau is functionally relevant, site-specific mutagenesis was utilized to replace potential zinc-binding sites (Mo et al., 2009) in the Tau* protein.

To determine whether this effect could be blocked by introducing mutations at K259 and K353, site-directed mutagenesis was utilized to generate a construct containing K to Q mutations, mimicking constitutive acetylation.

For site-directed mutagenesis, the following primers were utilized: Sebox 5 (new_mutant at position 2 generating the second G): 5′ GATGATGAACTC TTCACgTCCCACACG 3′; Sebox 6 (new mutant at position 3 generating the first E): 5′ GATGATGAACTCTTtACCTCCCACACG 3′; Sebox 7 (new_mutant at position 4 generating the second E): 5′ GATGATGAACTtTTCACCTCCCACACG 3′.