Sentence examples for mutagenesis were ordered from inspiring English sources

Primers for cloning and mutagenesis were ordered from Valuegene.

The QuikChange XL kit for site-directed mutagenesis was from Stratagene, and oligonucleotide primers were ordered from VBC-Biotech.

Orders were orders.

In order to better understand the interfacial activation mechanisms of YLLip2, structure guided site-directed mutagenesis were performed, mutants were constructed, kinetics parameters and lipase properties were detected.

Site-directed mutagenesis, random mutagenesis, and site-saturation mutagenesis were utilized to increase the OP-degrading activity of Dr-OPH.

In order to explore this unusual Y-type oxyanion hole, and to improve LipR performance, two modification strategies based on site directed or saturation mutagenesis were addressed.

Because the effect of the AOX1 5′UTR on protein expression is not known, site-directed mutagenesis was performed in order to decrease or increase the length of this region.

PTEN full length cDNA was cloned in a pCDNA 3.1 (Invitrogen, Life Technologies Ltd ,UK) expression vector and site-directed mutagenesis was performed in order to obtain the mutation under study (PTENAsp24Val) and the known pathogenic mutation Asn48Lys (PTENAsn48Lys) [ 9].

Site-directed mutagenesis was performed on pEGFP-WRN[ 40] in order to change amino acid residues of WRN, Ser-440, −467, −−478 and −1141, to alanine using the QuikChange II Site-Directed Mutagenesis Kit or the QuikChange Multi Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA, USA).

Point mutations were introduced into the cDNA coding for MMP-2 using directional mutagenesis: site-directed mutagenesis was performed using primers with nucleotide substitutions in order to change cystein in alanine at amino acid 60 and/or 65 and 233.

Site-directed mutagenesis was used to create combinations of each substitution, in order to assess their individual contributions.