Sentence examples for mutagenesis were generated from inspiring English sources
PCR products used for en passant mutagenesis were generated with polyacrylamide-gel electrophoresis-purified primers (Biomers, Ulm, Germany).
Images for the FadD13 model and the docking studies were prepared by using Pymol [58] whereas the images showing the active site and location of residues selected for mutagenesis were generated by using CastP [34] and VMD [59], respectively.
The SCRIB internal deletions and point mutagenesis were generated using PCR-based site-directed mutagenesis.
Mutants of D. tertiolecta subjected to random insertional mutagenesis were generated by transformation using pGreenII0000 plasmid with a bleomycin selection cassette.
Site-directed mutagenesis was generated by PCR and the identities of individual clones were verified by sequencing.
A 1Bx14 knockout mutant derived from an elite Chinese wheat variety Xiaoyan 54 through chemical mutagenesis was generated and characterized, providing an opportunity for identifying the biological function of 1Bx14[ 42].
Saturation mutagenesis libraries were generated using the QuikChange® mutagenesis strategy from Stratagene (USA).
By employing site-directed mutagenesis, 4 SULT1allozymesmes were generated, expressed, and purified.
MHV-68 vM2Y (with tyrosine residues at positions 120 and 129 mutated to phenylalanines) and vM2P2 (with proline residues at positions 158, 160, 163 and 167 mutated to alanines) viruses were generated by mutagenesis of the viral genome cloned as a bacterial artificial chromosome (BAC) [47], [48].
Mutations in Scl and Lmo2 cDNAs were generated by mutagenesis as directed by the manufacturer (QuikChange Lightning Multi Site-Directed Mutagenesis Kit; Agilent Technologies).
Respective MglAG21V, MglAQ82A, MglAR53A and MglBA68/72R, MglBR124/E127A and MglBA5 (A5=E14/R15/R124/E127/R131A) mutants were generated by mutagenesis PCR.
