Sentence examples for mutagenesis were designed from inspiring English sources
The primers for mutagenesis were designed using PrimerX (http://bioinformatics.org/primerx/) and synthesized by IDT DNA technologies (Coralville, IA).
Oligonucleotide sequences used for mutagenesis were designed using the PrimerX tool (http://www.bioinformatics.org/primerx/).org/primerx/
The primers used for mutagenesis were designed using the web-based program PrimerX (http://bioinformatics.org/primerx/) and synthesized by IDT DNA technologies (Coralville, IA).
Primers for each respective mutagenesis were designed using Stratagene's web-based QuickChange® primer design program http://www.stratagene.com/qcprimerdesigne (primer sequences are found in Additional file 1; Table S1).
In order to increase the expression level of BGL in E. coli, site-directed mutagenesis were designed and performed to optimize condons of BGL for E. coli expression system.
Primers for site-directed mutagenesis were designed using the PrimerX program (http://www.bioinformatics.org/primerx/) under the following conditions: melting temperature >78 °C and primer termination with guanine (G) or cytidine (C) for QuickChange site-directed mutagenesis kit (Additional file 1: Table S3).
A recombinant protein with cysteine residue by using site-directed mutagenesis was designed and then directly immobilized on Au surface without any chemical linker.
A second round of loop mutagenesis was designed, in the main, to increase the number of charged residues.
The primer used for mutagenesis was designed by using the Internet-based program Primer3 (http://frodo.wi.mit.edu/primer3/) and synthesized by IDT Integrated DNA Technologiess).
Two sets of mutagenesis primers were designed with a 4 bp deletion for Hand and Meis1 transcription factor binding sites.
Mutagenesis of a construct of SERT tagged with CFP and YFP at the N- and C-termini, respectively (C-SERT-Y), was performed using the QuikChange lightning kit (Agilent Technologies, Santa Clara, CA), and mutagenesis primers were designed according to the manufacturer's protocol.
