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Results of the mutagenesis were confirmed by DNA sequencing (University of Wisconsin, Biotechnology Center-Madison).
The results of mutagenesis were confirmed by sequencing clones with the following internal primer, K310R 5′-GCCTGCAGGCTCCTGTGCGT-3′.
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Each round of mutagenesis was confirmed by sequencing.
Mutagenesis was confirmed by full-length bi-directional sequencing.
Site-directed mutagenesis was confirmed using DNA sequence analysis with the following primers: pET24d-αB-crystallin forward 5'-GTCAACCTGGATGTGAAGCA-3' and pET24d-αB-crystallin reverse 5'-CATTCACTGGTGGGGAAACT 3'-CATTCACTGGTGGGGAAACT 3
The success of the mutagenesis was confirmed by sequencing (Fig. 1B), Southern blot, and polymerase chain reaction (PCR) analysis (Fig. 1C).
Mutagenesis was confirmed by the inability to amplify either open reading frame (ORF) by polymerase chain reaction (PCR), by identification of the kanamycin resistance gene from the suicide vector in the genome (by PCR and colony blot hybridization), and by sequencing of PCR products (data not shown).
The correct mutagenesis was confirmed by sequencing.
Mutagenesis was confirmed by DNA sequencing.
The successful mutagenesis was confirmed by Sanger sequencing.
Successful mutagenesis was confirmed by DNA sequencing; the recombinant plasmids are listed in Table 1.
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