Sentence examples for mutagenesis we mutated from inspiring English sources

By site-directed mutagenesis, we mutated two of these sites (ser 62 and ser 83) substituting the serine with an alanine (data not shown).

Furthermore, using constructed site-directed mutagenesis we mutated Cx43 phosphorylation sites, to investigate target(s) that is likely involved in the IP3R-associated signal transduction.

To evaluate the performance of new enzyme mixture in a point mutagenesis, we first tested its ability to mutate Tyr 66 in the mutant GFPuv to Asp.

By identifying the Ctf genes that are the most sensitive to random mutagenesis, we predicted that human orthologs of the yeast Ctf genes may represent CIN genes mutated in tumors.

To determine whether the G92D mutation is responsible for the loss of AI-2 production, we mutated the Asp-92 back to a glycine by site-directed mutagenesis.

Then we performed site-directed mutagenesis and mutated two guanine residues in the predicted SP-1 binding site in the promoter region of IL-8.

We used QuickChange mutagenesis to mutate these sites (Stratagene, Santa Clara, CA, USA).

To do so, we used site directed mutagenesis to mutate the FOXO1a binding site in the promoter of TXNIP.

To elucidate whether S279/282 phosphorylation affects the gap permeability in cardiomyocytes, we used site-directed mutagenesis to mutate S279 and S282 into alanine or aspartic acid in rat Cx43 gene (wt-Cx43).

We then performed site directed mutagenesis to mutate lysine 27 to an arginine residue using primers oLC1931 and oLC1932 using standard Stratagene protocol.

The M10 mutant was generated from the wildtype Trask by site directed mutagenesis to mutate arginine 368 to aspartate.