Sentence examples for mutagenesis we inserted from inspiring English sources

Using directed mutagenesis, we inserted two restriction sites (SpeI and XhoI), which allowed the insertion of STX-GFP in the multiple cloning site of pTrip-CMV-WPRE plasmid.

Using site-directed mutagenesis, we inserted several mutations into the seed sequence of miR-125b-1 miR-125b-1 miR-125b-1-4 3′-UTR.

Using site-directed mutagenesis, we inserted a highly characterized 9′ serine to leucine mutation into the second transmembrane (M2) region of α4, β3 and δ subunits that increases receptor sensitivity to GABA.

In order to evaluate the capacity of insulators to block insertional mutagenesis, we first inserted cassette 234-β-EGFP on chromosome 4 at site RL5 to determine if insertion of an expression cassette would activate the flanking genes.

We inserted clauses guaranteeing our editorial independence".

We inserted this reference.

We inserted (nts1-10).

Afterwards we inserted a peripherally inserted central line.

Authors' response: We insert the missing legends.

For the site directed mutagenesis we used the Quickchange Site Directed Mutagenesis Kit (NO. 200518, Stratagene).

To generate N- or C-terminal HA-tagged LILRB2-ICD, we performed site-directed mutagenesis to insert the HA and linker sequences (Q5 Site-Directed Mutagenesis Kit, NEB, Ipswich, MA).