To investigate the involvement of cytosine methylation in this mutagenesis process, we analyzed the methylation profile of p53 exon 5, in lung carcinoma.
To explain why 10% of colonies were non-fluorescent, we extracted plasmids from the four non-fluorescent colonies formed in the five-point site-directed mutagenesis experiment, and we analyzed them using electrophoresis and DNA sequencing (Additional file 1: Figure S4).
Furthermore, using a reverse mutagenesis approach, we analyze the role of mutated amino acids upon the physicochemical factors that govern solubility of e-gp41.
To determine whether GC→TA transversions induced by DinB-mediated bypass of 8-oxo-dG contributes to H2O2-induced mutagenesis in P. aeruginosa in vivo, we analyzed the sequence of the rpoB gene from representative RifR clones (see Materials and Methods).
In order to evaluate the effects of mutagenesis on the quality of L. starkeyi oil, we analyzed the composition of fatty acids from lipids produced by A1 and wild-type strain during these bioreactor fermentations.
In addition, we analyzed C. elegans sgRNAs with published mutagenesis efficiencies according to the Doench et al. (2014) algorithm.
We analyzed a mathematical model of in vitro evolution conducted by repetition of mutagenesis and selection processes.
We analyzed the mutation frequency of the F1 offspring in time bins after mutagenesis of the founders.
Previously, we analyzed the 1-kb espB gene of EPEC using the linker scanning mutagenesis kit from New England Biolabs, which is based on in vitro Tn 7 random insertions (4).
