Sentence examples for mutagenesis we altered from inspiring English sources
Using site-directed mutagenesis we altered Leu226 in the HA RBS of RGWF10 to Gln, creating the mutant WF10 (mWF10).
Using site-directed mutagenesis, we can alter the appearance of the phases that comprise a catalytic wave.
To test whether a catalytically competent form of MK-STYX could be generated, we altered its signature motif by site-directed mutagenesis to convert Phe-245 and Ser-246 into the histidine and cysteine residues respectively, which are characteristic of the signature motif of an active PTP family member.
"And we altered them beyond recognition".
A MC where the specific vTopo site was removed (MCns) was constructed from pMC454 using the following mutagenesis primers (altered nucleotides are shown in bold) to form pMC454I in a QuikChange mutagenesis kit according to the manufacturer's instructions:Mutations were confirmed by DNA sequencing.
Since the experimenter can control the mutagenesis directly by reducing the concentration or time of mutagenesis, we suggest that future phenotype sequencing experiments should use a substantially lower mutagenesis density than we employed, to boost the signal-to-noise ratio.
For the site directed mutagenesis we used the Quickchange Site Directed Mutagenesis Kit (NO. 200518, Stratagene).
Furthermore, Q169 mutagenesis altered the relative strength of hTERT-telomeric ssDNA interactions, which identifies Q169 as a novel residue in hTERT required for optimal primer binding.
CLOCK was initially discovered in a mutagenesis screen for altered circadian phenotypes in mice [1].
Another study using microarray analyses found that gamma-ray mutagenesis altered the transcriptome of rice more profoundly than a transgenic modification.
