Sentence examples for mutagenesis was generated from inspiring English sources
Site-directed mutagenesis was generated by PCR and the identities of individual clones were verified by sequencing.
A 1Bx14 knockout mutant derived from an elite Chinese wheat variety Xiaoyan 54 through chemical mutagenesis was generated and characterized, providing an opportunity for identifying the biological function of 1Bx14[ 42].
PCR products used for en passant mutagenesis were generated with polyacrylamide-gel electrophoresis-purified primers (Biomers, Ulm, Germany).
Images for the FadD13 model and the docking studies were prepared by using Pymol [58] whereas the images showing the active site and location of residues selected for mutagenesis were generated by using CastP [34] and VMD [59], respectively.
The SCRIB internal deletions and point mutagenesis were generated using PCR-based site-directed mutagenesis.
Mutants of D. tertiolecta subjected to random insertional mutagenesis were generated by transformation using pGreenII0000 plasmid with a bleomycin selection cassette.
The mutagenesis PCR was generated by using OxyRcomF and OxyRcomR [39], and ligated to pMD18 T-Easy vector (Takara), designed as pMD18oxyRsd.
After three rounds of mutagenesis, a mutant was generated that decreased the biocatalyst loading 3-fold.
A Pbs2 random mutagenesis library (9×104 mutants) was generated with a Stratagene kit and cloned into the pRS416-GPD1 vector.
The expression vector for the mutant PheH R270K was generated by mutagenesis of pERPH2.0 using Pfu polymerase and the QuikChange mutagenesis protocol.
The mutant LynY397F cDNA was generated by mutagenesis of the MT-LynWT construct using the quickchange site-directed mutagenesis system (Stratagene, La Jolla, CA) according to the manufacturer's recommendations.
