Sentence examples for mutagenesis utilizing a from inspiring English sources

The vector encoding wild-type Myc was used as a template for oligonucleotide directed point mutagenesis utilizing a QuickChange II kit according to the directions of the supplier (Stratagene, Inc. LaJolla CA).

PIPE mutagenesis utilizing overlapping complementary 5′ primer ends was used to amplify mutant constructs for all mutants.

Utilizing a site-directed mutagenesis approach, two scFv43 revertants were constructed: scFv43 M2 and M1.

By utilizing a transposon-based mutagenesis approach, we isolated a swarm-defective mutant of S. typhimurium (21).

Utilizing a site-directed mutagenesis approach and accessibility measurements by EPR spectroscopy, Dong et al. [ 21] showed that TM helix 2 is in direct contact with the lipid bilayer.

The mutant transporters were constructed utilizing a Stratagene Quickchange XL site-directed mutagenesis kit.

Utilizing a commercially acquired GPS®-linker scanning (LS) mutagenesis system, random insertions of 5 amino acids or truncations were introduced into the coding sequence of the plasmid-borne levQ gene, generating a library of clones of levQ mutants.

Nipponbare utilizing a deep sequencing approach.

Utilizing an identified and publicly available mutant line (termed neb) in the nebulin gene generated by ENU mutagenesis at the Sanger Institute Zebrafish Mutant Resource, we have defined the behavioral, morphological, functional and histopathological features of the neb zebrafish.

Therefore, for these developmental stages we utilized a traditional bisulfite mutagenesis approach to analyze the DNA methylation status at 36 CpG sites, including all 29 sites contained within the Dlk1 CpG island and all 16 sites analyzed using the hairpin linker employed for analysis of DNA derived from older embryonic, neonatal, and adult tissue.

The first phase utilized a single round of random mutagenesis and screening in bacterial lysates with coelenterazine for brighter luminescence.