Sentence examples for mutagenesis using an from inspiring English sources

In this study, an improved method for saturation mutagenesis using an amber codon was developed.

The resulting DNA was subjected to bisulfite mutagenesis using an EZ DNA methylation-direct kit (Zymo Research, cat# D5020).

The T3/Y291F mutant carries the mutation of Tyr291 to phenylalanine (F291) and was constructed by means of site-directed mutagenesis using an in vitro oligonucleotide mutagenesis system (Altered Sites in vitro Mutagenesis System, Promega).

A total of 0.5 μg of hairpin linked-ligated DNA was denatured by incubating in freshly prepared 3 M NaOH for 20 min at 42°C, then subjected to bisulfite mutagenesis using an EZ DNA methylation-direct kit, as above.

A plasmid to express the T238A mutation of Tub2p (yeast β-tubulin) was made by QuikChange (Stratagene) mutagenesis, using an expression plasmid for wild-type Tub2 as template and with primers designed according to the manufacturer's instructions.

Here, we demonstrate a method for the rapid production of negative control tandem scFvs through complementarity determining region (CDR) mutagenesis, using a recently described bispecific T-cell engager (BiTE) targeting a tumor-specific mutation of the epidermal growth factor receptor (EGFRvIII) as an example.

This mutation was generated by site-directed mutagenesis using a QuickChange kit (Stratagene, La Jolla, CA).

Further mutations were introduced into pGPfs by site directed mutagenesis using a Stratagene QuikChange Multi Site-Directed Mutagenesis Kit.

A set of complementary oligonucleotides containing the desired mutations was synthesized (Table 1) and used as primers for site-directed mutagenesis using a Quickchange kit (Stratagene, La Jolla, CA).

All topoIIβ constructs containing the S165R mutation were changed to give wild type sequence by site directed mutagenesis using a Chameleon kit (stratagene) according to manufacturer's instructions.

Dnmt1 RFM mutants were generated by site-directed mutagenesis using a plasmid in which the Dnmt1 cDNA is transcribed from the mouse Pgk1 promoter [16].