Sentence examples for mutagenesis using a from inspiring English sources
Here, we demonstrate a method for the rapid production of negative control tandem scFvs through complementarity determining region (CDR) mutagenesis, using a recently described bispecific T-cell engager (BiTE) targeting a tumor-specific mutation of the epidermal growth factor receptor (EGFRvIII) as an example.
This mutation was generated by site-directed mutagenesis using a QuickChange kit (Stratagene, La Jolla, CA).
Further mutations were introduced into pGPfs by site directed mutagenesis using a Stratagene QuikChange Multi Site-Directed Mutagenesis Kit.
All topoIIβ constructs containing the S165R mutation were changed to give wild type sequence by site directed mutagenesis using a Chameleon kit (stratagene) according to manufacturer's instructions.
Dnmt1 RFM mutants were generated by site-directed mutagenesis using a plasmid in which the Dnmt1 cDNA is transcribed from the mouse Pgk1 promoter [16].
The M. tuberculosis oppD mutant was constructed by targeted mutagenesis using a temperature sensitive-sacB delivery system as described previously [22].
In this study, an improved method for saturation mutagenesis using an amber codon was developed.
The resulting DNA was subjected to bisulfite mutagenesis using an EZ DNA methylation-direct kit (Zymo Research, cat# D5020).
The T3/Y291F mutant carries the mutation of Tyr291 to phenylalanine (F291) and was constructed by means of site-directed mutagenesis using an in vitro oligonucleotide mutagenesis system (Altered Sites in vitro Mutagenesis System, Promega).
A total of 0.5 μg of hairpin linked-ligated DNA was denatured by incubating in freshly prepared 3 M NaOH for 20 min at 42°C, then subjected to bisulfite mutagenesis using an EZ DNA methylation-direct kit, as above.
A plasmid to express the T238A mutation of Tub2p (yeast β-tubulin) was made by QuikChange (Stratagene) mutagenesis, using an expression plasmid for wild-type Tub2 as template and with primers designed according to the manufacturer's instructions.
