Sentence examples for mutagenesis scanning from inspiring English sources

Next, PEGylation of xGLP-3 was performed at specific sites, which were determined using cysteine mutagenesis scanning.

DOI: http://dx.doi.org/10.7554/eLife.06120.011 10.7554/eLiFigure20.012 Figure 4 figure supplement 1. Mutagenesis scanning for the phosphorylation site of SUMO-AMBN-N.

Aβ single-point proline mutagenesis: scanning mutagenesis studies have been performed on Aβ40 to detect the sequence position effect of proline-, alanine- and cysteine-replacement on amyloid fibril formation, measured by WT/mutant ΔΔG (Shivaprasad et al., 2006; Williams et al., 2004, 2006).

In the case of unnatural amino acid incorporation and mutagenesis scanning, EC50 values were obtained using a hypersensitive mutation in the α4 subunit (L9′A).

We isolated functional fragments of three of the LkARSs (LkARS-E139, LkARS-E848, and LkARS-C35) that are shorter than 100 bp and performed mutagenesis scanning experiments to identify specific sequences necessary for the ARS function of these DNA fragments.

To investigate the possible mechanisms of subcellular localisation of Irgm1, an alanine mutagenesis scan was performed based on the targeting construct EGFP-Irgm1 αK.

In this study we have carried out a mutagenesis scan of the first 41 residues of the putative mature CLR sequence (residues 23 63) in order to identify potential binding sites for non-peptidic antagonists.

4) "Big data" mutagenesis scans must sacrifice something in their broad scope; for example, quantitative as opposed to binary fitness measurements, intragenic epistasis (e.g. interactions among substitutions within HA as will occur in this experimental set-up), pool drop out bottlenecks.

Although a number of residues identified in the functional mutagenesis scan do not contribute to the predicted TPenA binding site in this particular model, they are not predicted to contribute to the binding site in any of the other models (Supplementary Table S1).

In order to confirm the direct role of CLR in antagonist binding and to locate the non-peptide-binding site more precisely, we carried out a mutagenesis scan of the first 41 residues of the putative mature CLR sequence (residues 23 63).

This includes an analysis of the 289-residue HET-s/HET-S natural homologs found in P.anserina, a combination of three Aβ scanning mutagenesis studies, and a set of 74 synthetic mutants of Aβ created by random mutagenesis.