Sentence examples for mutagenesis or expression from inspiring English sources

Traditional methods for genetic manipulation of poxviruses, either for mutagenesis or expression of exogenous genes, rely on onerous plaque purification of rare recombinant viruses.

Further mutagenesis or expression analysis using these sequence variants may confirm a role for these amino acid residues in determining significant phenotypic effects, similar to differences seen in nature among the species involved.

No vector was detected in the tumor cells of five animals with malignancies, indicating that the malignancies were not caused by insertional mutagenesis or MGMTP140K expression.

In the future further functional analysis through mutagenesis or mis-expression of those microRNAs specifically enriched during the process of male or female gametogenesis should reveal candidate genes whose disruption could block mosquito reproduction.

Smaller projects designed to develop novel techniques, such as strategies for mutagenesis, conditional gene expression, or for identifying new genes or mutants, as well as new tools to mine and analyze sequence data.

The tsA201 cells were transfected with WT or mutant rNav1.4 cDNA contained in the mutagenesis and expression vector pAlter-Max (Promega) (10 ug) [73] along with human β1-subunit (10 ug) cDNA utilizing the calcium phosphate method and grown to 40 50% confluence on 100 mm plates [72], [74].

This type of transgene delivery has disadvantages, such as the risk of insertional mutagenesis, residual expression of integrated transgenes, and uncontrolled activation or inactivation of the integrated transgenes, which is critical in the case of iPSC reprogramming, since all four of the pluripotency factors are oncogenic (Hu, 2014).

To further establish the role of 15-LO1 in regulating HIF-1 α expression, the site-directed mutagenesis or truncation of human 15-LO1 was carried out to attenuate 15-LO1 function.

Previous in vitro mutagenesis and expression of these modifications (G137D, in the oxyanion hole and W251L, in the acyl pocket) have confirmed their functional significance.

The mutagenesis and expression of β-Lactamase and BLIP and conjugation to oligonucleotides have been reported by Janssen et al.34 For the labeling of the β-lactamase- and BLIP-DNA conjugates for STORM imaging, the proteins were buffer exchanged to PBS (100 mM sodium phosphate, 150 mM NaCl, pH 7.2) using Zeba spin desalting columns (7k MWCO, 0.5 mL, Thermo Fisher).

By combining site-directed mutagenesis, heterologous expression, and molecular dynamics simulations that capture the conformational change of constitutively active mutants, we tentatively identified crucial residues for the function of these receptors using the mouse MOR256-3 (Olfr124) as a model.