Sentence examples for mutagenesis of two from inspiring English sources

Systematic mutagenesis of two major BoNT/A binding pockets was conducted in order to adapt these pockets to corresponding amino acids of human SNAP-23.

Here we evolved improved transketolase activities towards aromatic aldehydes, by saturation mutagenesis of two active-site residues (R358 and S385), predicted to interact with the aromatic substituents.

Except for mutagenesis of two conserved residues, there has been no structure-function analysis of Hhat, and the regions of the protein required for Shh palmitoylation are unknown.

Site directed mutagenesis of two residues located at either side of the pocket caused distinct resistance to the compounds, demonstrating that they indeed bind at this site.

In an attempt to shed additional light on this latter possibility, we undertook extensive mutagenesis of two of the newly-identified CRMs, notum_cooc310 and jhamt_cooc102.

Site-specific mutagenesis of two identified stress resistant mRNAs (PPP1R15B and IFRD1) demonstrated that a single uORF is sufficient for eIF2-mediated translation control in both cases.

Site-directed mutagenesis of eight cysteines was used to disrupt disulfide bonds to generate molecules with altered IgE-binding capacity.

Strikingly, mutagenesis of three different hydrophobic residues within the putative NES, namely M144, L147, and I149, completely impaired cytosolic relocalization of TFEB upon refeeding, indicating that the integrity of the NES sequence is required for TFEB nuclear export (Fig. 3c, d).

The well-established non-allergenic properties of BB18 were used to generate a genetic variant of Ole e 1, named OB55 58, by site-direct mutagenesis of four residues (E55V56G57Y58) in an IgE/IgG epitope of Ole e 1 by the corresponding ones in BB18 (SDSE).

Moreover, site-directed mutagenesis of four conserved cysteines in Fe-S cluster of yeast XPD (Rad3) leads to defects in excision repair of UV photoproducts (Wu et al., 2009).

Site-specific mutagenesis of eight Asn-linked glycan consensus sites (Asn to Gln mutations) suggested that PLD4 was exogenously expressed in HEK293 cells and contained large N-glycosylated moieties at multiple consensus sites (Fig. S5B).