Sentence examples for mutagenesis of specific from inspiring English sources

DNA cleavage enzymes can be used to induce targeted mutagenesis of specific genes, including those of exogenous viruses.

Mutagenesis of specific residues was carried out using the QuikChange XL site-directed mutagenesis kit (Stratagene) as per manufacturer's instructions.

Mutagenesis of specific protein binding sites had no effect on DNA methylation imprint establishment at the ICR: methylation was undisturbed in mutant male germ cells and lack of methylation was undisturbed in mutant female germ cells [45], [58], [59], [60].

In the case of the binding site for IL-11Rα or site I, directed mutagenesis of specific residues at the C-terminus of helix D and the AB loop established that these regions participate in receptor binding and cytokine-mediated bioactivity [17] [21], analogous to IL-6 [39].

These studies, together with the analysis of the activity of the protein following in vitro mutagenesis of specific residues, provide circumstantial support for a reaction mechanism involving a quinone-methide intermediate and the participation of a key lysine residue in an unusual push-pull like two-step catalytic mechanism.

Directed mutagenesis of specific loci in the genome would greatly speed up analysis of gene function.

Therefore, NNK was commonly used for the site-saturation mutagenesis of a specific amino acid (Tian et al. 2013).

Using site-directed mutagenesis of RyR2, a specific Ca2+/calmodulin-dependent protein kinase II (CaMKII) phosphorylation site (Ser) distinct from the PKA phosphorylation site (Ser) has been identified [ 6].

Nucleotide sequence contexts of somatic mutations can be indicative of specific mutagenesis mechanisms occurring in tumor cells [ 54– 54].

Through these rounds of mutagenesis enzyme specific activity improved from 5.9 to 1582.8 IU/g with an overall improvement in product yield due to reduced biocatalyst loading.

However, it is worth noting that Sfp2 was identified in that Spanish isolate by shaving the surface of bacteria with proteases followed by LC/MS/MS analysis of the resulting peptides and homology comparisons, and not by the use of mutagenesis and specific antibodies directed against that protein [38].