Sentence examples for mutagenesis of putative from inspiring English sources

Mutagenesis of putative ets binding sites (EBS) in the DKK2 5'upstregionegion was performed using KOD-plus (TOYOBO).

The involvement of caspases in BAG3 degradation was established by protection of BAG3 in the presence of the pan-caspase inhibitor zVAD and by mutagenesis of putative caspase recognition sites.

We next conducted site-directed mutagenesis of putative N-glycosylation sites of PCX to examine their possible effects on the extracellular PCX activity.

Site-directed mutagenesis of putative FOXO binding sites was performed using a QuikChange site-directed mutagenesis kit (Stratagene, La Jolla, CA, USA).

Mutagenesis of putative PKC phosphorylation sites within the C-terminal domain of HCN2 revealed that dephosphorylation of Thr was critical for the prolonged Ca2+ entry required for AIF-mediated apoptosis.

Using this metabolite in vitro as a substrate analogue, SalBIII has been shown to form pyran ring A. We have determined the X-ray crystal structure of SalBIII, and structure-guided mutagenesis of putative active-site residues has identified Asp38 and Asp104 as an essential catalytic dyad.

Open image in new window Fig. 3 Mutagenesis of DGAT1 putative phosphorylation sites and TAG synthetic activities.

In this study, we characterize the in vivo role of the Fe-S cluster binding domain in Pri2 function by systematic site-directed mutagenesis of the putative cysteine ligands.

Surprisingly, mutagenesis of these putative phosphorylation sites (including those matching consensus sequences for CDK or PAK family kinases and those not) has not resulted in any observable defects in cell polarization and growth by a variety of assays, suggesting that phosphorylation is largely dispensable for the regulation of Cdc24.

Site-directed mutagenesis of the putative C/EBP α binding sites decreased FTO promoter activity.

Site-directed mutagenesis of a putative proton-binding residue abolished transport without affecting signaling.