Sentence examples for mutagenesis of four from inspiring English sources

Moreover, site-directed mutagenesis of four conserved cysteines in Fe-S cluster of yeast XPD (Rad3) leads to defects in excision repair of UV photoproducts (Wu et al., 2009).

The well-established non-allergenic properties of BB18 were used to generate a genetic variant of Ole e 1, named OB55 58, by site-direct mutagenesis of four residues (E55V56G57Y58) in an IgE/IgG epitope of Ole e 1 by the corresponding ones in BB18 (SDSE).

It is often assumed that mutagenesis of four phenylalanine residues (F305L, F341L, F359L, and F368L) within the RRM of human FUS effectively abolishes interactions between FUS and RNA.

We further detected the function of PcNLPs protein in vitro based on the site directed mutagenesis of four amino acid residues in a conserved motif.

Mutagenesis of four conserved residues previously reported to be involved in urate binding to Deinococcus radiodurans HucR and Rhizobium radiobacter PecS significantly reduced protein stability and DNA binding affinity but not ligand binding.

Our structure of the dynein Lis1 complex shows that the Lis1 β-propeller contacts dynein primarily at a surface-exposed helix at the junction of AAA3 and AAA4, explaining why mutagenesis of four conserved, charged residues (KDEE) on this helix virtually abolished Lis1 binding and dynein regulation (Huang et al., 2012).

Site-directed mutagenesis of eight cysteines was used to disrupt disulfide bonds to generate molecules with altered IgE-binding capacity.

Systematic mutagenesis of two major BoNT/A binding pockets was conducted in order to adapt these pockets to corresponding amino acids of human SNAP-23.

Here we evolved improved transketolase activities towards aromatic aldehydes, by saturation mutagenesis of two active-site residues (R358 and S385), predicted to interact with the aromatic substituents.

Except for mutagenesis of two conserved residues, there has been no structure-function analysis of Hhat, and the regions of the protein required for Shh palmitoylation are unknown.

Site directed mutagenesis of two residues located at either side of the pocket caused distinct resistance to the compounds, demonstrating that they indeed bind at this site.