Sentence examples for mutagenesis of each from inspiring English sources

Sublibraries created by site-specific randomization mutagenesis of each single site were screened using a spectroscopic assay based on ω-p-nitrophenoxycarboxylic acids (pNCA).

Oligonucleotide mutagenesis of each open reading frame and maxicell analysis demonstrated that all open reading frames are expressed in vivo.

Future studies involving systematic mutagenesis of each Trp residue may shed more light on the properties of this unusual feature of the CD spectrum.

In an attempt to assign functionality, we performed site directed mutagenesis of each of these residues to an alanine, in addition to constructing the double mutant H115A/R138A.

Mutagenesis of each PPRE site or of the two sites suppressed the FBS and WY induction but not the 3-MC-mediated CYP1A1 induction (Figure 3B).

Mutagenesis of each cysteine in TNFα showed that Cys49 was uniquely responsible for the crosslinks.

Although various methods have been developed for ligating DNA molecules or targeted mutagenesis of plasmids, each has its limitations.

Three variants of both Mal d 1 and Cor a 1 were generated based on in silico mutagenesis of models of each WT allergen using an algorithm, which has been successfully applied to modify Bet v 1.0101.

Experimental characterization of the paired YdgE/YdgF protein complex in E. coli has revealed its involvement in spermidine excretion and confirmed that only YdgE (MdtI) was essential for transport activity based on site-directed mutagenesis experiments of each active site Glu14 residue in the pair [ 11].

The Kudzu EFE contains 10 His residues, and when site-directed mutagenesis of His to Gln of each was carried out, this resulted in 0 to 60% of activity compared with wild type [ 32].

In order to prove that these mutations were responsible for the disease state, each of the three mutations was constructed separately by site-directed mutagenesis of the normal PNP cDNA, and each was transiently expressed in COS cells.