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Peptide nucleic acid-targeted mutagenesis of a chromosomal gene in mouse cells.
(A) Saturation mutagenesis of a LAT peptide with screening for phosphorylation by ZAP-70, with blue corresponding to low phosphorylation.
In vitro mutagenesis of a full-length cDNA clone of Semliki Forest virus: the 6,000-molecular-weight 6,000-molecular-weight 6,000-molecular-weight
Site-directed mutagenesis of a cryptic glycosylation motif not previously identified allowed production of rhodesain suitable for crystallization.
The 58 amino acid SPA-binding affinity tag ZSPA-1, was previously selected from a library constructed by combinatorial mutagenesis of a protein domain from SPA.
Here we have analyzed the effect on rat PAH enzyme kinetics of in vitro mutagenesis of a number of these amino acids lining the PAH active site.
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A dwarf mutant line (bnaC.dwf) was obtained by chemical mutagenesis of an inbred line T6.
For instance, an amine dehydrogenase that allows for the direct asymmetric amination of ketones with ammonia was created by mutagenesis of an l-amino acid dehydrogenase.
Site-directed mutagenesis of an α-amylase isolated from Bacillus megaterium WHO has been performed to evaluate the roles of the calcium binding site residues in enzyme thermostability.
Very recently, mutagenesis of an eye development gene was achieved in one congener Daphnia magna using the CRISPR/Cas system [ 23].
Further optimization by random mutagenesis of an EG gene expressed in S. cerevisiae resulted in increased thermostability and extracellular activity [ 106].
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