Sentence examples for mutagenesis methods for from inspiring English sources
Many laboratories use alternative physical or chemical mutagenesis methods for A. nidulans, including UV light and MNNG.
H3.3 S57andnd H3.3 T32I cDNAs were generated by the site-directed mutagenesis method for the H3f3a cDNA template.
The results shed light on α-KG production in eukaryotic cells, and pave the way for a high-throughput screening and random mutagenesis method for enhancing α-KG production.
We report here the development of a random mutagenesis method for directed evolution of Streptomyces lividans acetyl xylan esterase (AxeA), which we previously showed is able to deacetylate chitinous substrate, in order to obtain chitooligosaccharides with well-defined structural properties.
We previously reported a four-base codon-mediated saturation mutagenesis method for the expression of all 20 amino acid-substituted proteins from one four-base codon-containing gene using 20 types of chemically aminoacylated tRNAs corresponding to the four-base codon.
The results demonstrated that in situ error-prone PPCP is an efficient random mutagenesis method for directed evolution of genes and proteins.
New mutagenesis methods developed for random mutagenesis, focused mutagenesis and DNA recombination were surveyed.
With the purpose of furthering our understanding of the enzymatic mechanism for lignin degradation by Pleurotus species, the above peroxidase genes from the P. ostreatus genome have now been heterologously expressed, structurally characterized using crystallographic and site-directed mutagenesis methods, and evaluated for their activity on lignin and related substrates.
Using the parameters defined in this study, the evaluated mutagenesis methods can be used for controlled mutagenesis, where the intended average frequency of induced mutations can be adjusted to a desirable level.
that closely resembles the previously described folding reporter GFP [24] as a starting point for mutagenesis (Methods S1).
The mutagenesis method used for this rol-3 suppressor screen was modified from (Flibotte et al. 2010).
